global® total® LGGT 單一階段連續性培養液 (含蛋白質）

Indications for Use

Culture of human embryos from zygote to blastocyst, embryo transfer.

Storage and Shelf Life

Store at 2-8°C and protected from light. Ten (10) weeks from the date of manufacture.

Disposal Consideration

Treat or dispose of waste material in accordance with all local state/provincial, and national requirements. Dispose with laboratory waste.

Composition

A bicarbonate-buffered protein-supplemented medium replete with glucose, lactate, pyruvate and all 20 amino acids is optimal to support the growth and development of human embryos in vitro.

Sodium Chloride, Sodium Pyruvate, L-Arginine, L-Threonine, Potassium Chloride, L-Alanine, L-Cystine, L-Tryptophan, Calcium Chloride, L-Asparagine, L-Histidine, L-Tyrosine, Potassium Phosphate, L-Aspartic Acid, L-Isoleucine, L-Valine, Magnesium Sulfate, L-Glutamic Acid, L-Leucine, Glycyl-L-Glutamine, Sodium Bicarbonate, Glycine, L-Lysine, EDTA, Glucose, L-Proline, L-Methionine, Phenol Red, Sodium Lactate, L-Serine, L-Phenylalanine, Human Serum Albumin* (4.4 mg/ml), Human α- and β-globulins* (0.6 mg/ml), Gentamicin Sulfate* (10 µg/ml)

*from therapeutic-grade source material

QUALITY CONTROL SPECIFICATIONS

Special Note on the CO2 Concentration in the Incubator: In most cases, a 5-7% concentration of CO2 in the incubator will produce a pH of 7.2 to 7.4 in global® total®. However, the exact concentration of CO2 required to produce the optimum pH of approximately 7.30 (7.27-7.33) depends on several factors, including the physical characteristics of incubator and the altitude. Consequently, we strongly recommend that each laboratory determine and use the concentration of CO2 that is required to produce a pH of 7.30 in global® total®.

INSTRUCTIONS FOR USE

The procedures described below have been found to be effective for the culture of human embryos from zygote to blastocyst, embryo transfer and are offered only as examples. Every laboratory must define and optimize its own procedures.

1. Prepare culture dishes containing 25-100 µl droplets or in larger volumes (0.5-1.0 ml) of global® total® under oil, according to general laboratory practice.
2. Before introducing the embryos, place the culture dishes in the incubator for sufficient time to ensure CO2 and temperature equilibration. Depending on the exact configuration, this may take from 24-48 hours. Equilibration will require less time if the oil and medium have been preequilibrated.
3. On Day 1, place the zygotes into the equilibrated global® total®. Culture the embryos for 48 h (Day 3, 4-8 cell stage).
4. For further culture to the blastocyst stage:

either a) transfer the cleavage-stage embryos to fresh medium under fresh oil and return to the incubator

or   b) maintain the embryos in the same medium (See Reed et al., 2009; 2010). Note that such uninterrupted culture requires special attention to air quality.