global® Collect® HEPES-buffered medium
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37°C 下使用的HEPES 緩衝培養基，用於取卵時cumulus-oocyte complexes的搜尋、保存與清洗。
需另加Protein Supplement
Indications for Use
Oocyte retrieval and washing.
Storage and Shelf Life
Store at 2–8 °C and protected from light. Ten (10) weeks from the date of manufacture.
Composition
A HEPES-buffered medium is required when oocytes are handled outside of a CO2 incubator.
Sodium Chloride, Potassium Chloride, Calcium Chloride, Potassium Phosphate, Magnesium Sulfate, Sodium Bicarbonate, Glucose, Lactate Na Salt, Sodium Pyruvate, Glycine, L-Alanine, L-Arginine HCl, L-Asparagine, L-Aspartic Acid, L-Cystine, L-Glutamic Acid, Glycyl-Glutamine, L-Histidine, L-Isoleucine, L-Leucine, L-Lysine HCl, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine, L-Valine, EDTA, Phenol Red, HEPES, Gentamicin Sulfate* (10 µg/ml) *from therapeutic-grade source material
QUALITY CONTROL SPECIFICATIONS
|
Assay (performed for each batch) |
Specification |
|
Physicochemical Tests |
|
|
pH (at room temperature) |
7.4-7.45 |
|
pH (at 37°C) |
7.2-7.4 |
|
Osmolality |
260-270 mOsM |
|
Biological Tests |
|
|
Endotoxin (LAL) |
≤ 0.5 EU/ml |
|
Sterility Test (bacterial and fungal screen, SAL 10–3) |
PASS |
|
Biological Assays |
|
|
1-cell Mouse Embryo Assay (% expanded blastocysts at 96 h of culture after 1 h exposure) |
≥ 80% |
INSTRUCTIONS FOR USE
The procedures described below have been found to be effective for the retrieval and washing of human oocytes and are offered only as examples. Every laboratory must define and optimize its own procedures.
- global® Collect® should be supplemented with Human Serum Albumin (HSA) or LifeGlobal® Protein Supplement to achieve desired % (v/v) of protein supplementation.
- Warm the protein-supplemented global® Collect® to 37°C prior to use.